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Data Tables

Lifetime Data of Selected Fluorophores

The following data tables provide you with information on the fluorescent lifetimes, excitation and emission wavelengths of Selected fluorescent dyes, probes and labels that are frequently used for biological applications and in biomedical research.

Fluorophore Lifetime [ns] Solvent Excitation max [nm] Emission max [nm]
5-Hydroxytryptamine     370-415 520-540
ATTO 565 3.4Water561585
ATTO 655 3.6Water655690
Acridine Orange 2.0PB pH 7.8 500530
Acridine Yellow     470 550
Alexa Fluor 488 4.1PB pH 7.4494519
Alexa Fluor 532     530 555
Alexa Fluor 546 4.0PB pH 7.4554570
Alexa Fluor 633 3.2Water621639
Alexa Fluor 647 1.0Water651672
Alexa Fluor 680 1.2PB pH 7.5682707
BODIPY 500/510     508 515
BODIPY 530/550     534 554
BODIPY FL 5.7Methanol502510
BODIPY TR-X 5.4Methanol588616
Cascade Blue     375 410
Coumarin 6 2.5Ethanol460505
CY2     489 506
CY3B 2.8PBS558572
CY3 0.3PBS548562
CY3.5 0.5PBS581596
CY5 1.0PBS646664
CY5.5 1.0PBS675694
Dansyl     340 520
DAPI 0.16 TRIS/EDTA341496
DAPI + ssDNA 1.88TRIS/EDTA358456
DAPI + dsDNA 2.20TRIS/EDTA356455
DPH     354 430
Erythrosin     529 554
Ethidium Bromide - no DNA 1.6TRIS/EDTA510595
Ethidium Bromide + ssDNA 25.1TRIS/EDTA520610
Ethidium Bromide + dsDNA 28.3TRIS/EDTA520608
FITC 4.1PB pH 7.8494518
Fluorescein 4.0PB pH 7.5495517
FURA-2     340-380 500-530
GFP 3.2Buffer pH 8498516
Hoechst 33258 - no DNA 0.2TRIS/EDTA337508
Hoechst 33258 + ssDNA 1.22TRIS/EDTA349466
Hoechst 33258 + dsDNA 1.94TRIS/EDTA349458
Hoechst 33342 - no DNA 0.35TRIS/EDTA336471
Hoechst 33342 + ssDNA 1.05TRIS/EDTA350436
Hoechst 33342 + dsDNA 2.21TRIS/EDTA350456
HPTS 5.4PB pH 7.8454511
Indocyanine Green 0.52Water780820
Laurdan     364 497
Lucifer Yellow 5.7Water428535
Nile Red     485 525
Oregon Green 488 4.1Buffer pH 9493520
Oregon Green 500 2.18Buffer pH 2503522
Oregon Green 514     511 530
Prodan 1.41Water361498
Pyrene > 100 Water341376
Rhodamine 101 4.32Water496520
Rhodamine 110 4.0Water505534
Rhodamine 123     505 534
Rhodamine 6G 4.08Water525555
Rhodamine B 1.68Water562583
Ru(bpy)3[PF6]2 600Water455605
Ru(bpy)2(dcpby)[PF6]2 375Buffer pH 7458650
SeTau-380-NHS 32.5 Water 270 480
SeTau-404-NHS 9.3 Water 402 515
SeTau-405-NHS 9.3 Water 405 518
SeTau-425-NHS 26.2 Water 340
425

545
SITS     336 438
SNARF     480 600-650
Stilbene SITS, SITA     365 460
Texas Red 4.2Water589615
TOTO-1 2.2Water514533
YOYO-1 no DNA 2.1TRIS/EDTA457549
YOYO-1 + ssDNA 1.67TRIS/EDTA490510
YOYO-1 + dsDNA 2.3TRIS/EDTA490507
YOYO-3     612 631

PBS = phosphate buffered saline pH 7.4
PB = phosphate buffer
TRIS/EDTA (1mM, pH 7.4) = tris(hydroxymethyl)aminomethane/ethylenediamine-tetraacetic acid.
ss = single-stranded
ds = double-stranded