Overview of Parallel 16-PMT Spectral FastFLIM
Spectral FLIM adds the fluorescence spectrum and lifetime (λ, τ) dimensions to the confocal scanning microscopy dimensions (x, y, z, t) resulting in a six-dimensional data set. We present a new time-resolved spectral detector with parallel 16-channel digital frequency domain FLIM (FastFLIM) readouts for fast spectral FLIM data acquisition. The 16-channel FastFLIM can produce unbiased spectral FLIM data for phasor analysis that unambiguously discriminates and quantitates fluorescent species with unique spectral and lifetime features at each image pixel. Our spectral FLIM method offers new opportunities for monitoring multiple dynamic signaling events in live specimens providing insights into complex biological systems.
Compatible with Alba STED, Alba v5, Q2, PL1, Evident (Olympus), Nikon, and Zeiss.
Product Specifications for Parallel 16-PMT Spectral FastFLIM
Sixteen-channel linear array multianode PMT characteristics
Number of individual detection channels (photocathodes)
- 16
Photocathode type
- GaAsP
Detection wavelength range
- 300 - 740 nm
Peak quantum efficiency wavelength
- 520 nm
Peak quantum efficiency
- 45%
Gain adjustment
- 0.01 to 1 in 256 steps for each photocathode
Transit time spread (TTS)
- < 300 ps
Spectrograph and fiber bundle
| Groove Density (grooves/mm) | Linear Dispersion (nm/mm) | Spectral Width (nm) |
|---|---|---|
| 600 | 13.6 | 213 |
| 900 | 8.7 | 139 |
| 1200 | 6.3 | 99 |
| 300 | 28 | 441 |
Fiber bundle input diameter
- 1.2 mm
Sixteen-channel parallel FastFLIM data acquisition characteristics
Number of independent data acquisition channels
- 16
Data transfer
- 4 x USB 3.0
CLK frequency
- 640 MHz
Decay times measurement range
- 100 ps - 100 ms
External laser CLK synchronization
- 10 - 80 MHz (LVTTL/TTL, 50 Ω)
Image scanning synchronization
- Pixel/Line/Frame clock inputs
Raw data size
- 32 bits
Raw data file structure
- Binary file with a header of 256 bytes
Max counting rate
- 160 x 106 counts/s (107 counts/s/channel)
Unit
Dimensions (cm)
- 45 x 24 x 44
Weight (kg)
- 15
Operating voltage
- 100 - 220 VAC, 50/60 Hz
Operating ambient temperature
- 18 - 30 °C
Example Configuration for Parallel 16-PMT Spectral FastFLIM
Product Resources
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“Characterization of a time-resolved spectral detector for spectral fluorescence lifetime imaging with the parallel 16-channel FastFLIM and the phasor analysis.” Sun, Y., Nguyen, T.D., Chen, Y.-I., Coskun, U.C., Liao, S.-C. & Yeh, H.-C. Multiphoton Microscopy in the Biomedical Sciences {XXIII}, 2023, Apr. doi: 10.1117/12.2656271. -
“Phasor S-FLIM: a new paradigm for fast and robust spectral fluorescence lifetime imaging.” Scipioni, L., Rossetta, A., Tedeschi, G. & Gratton, E. Nature Methods, 18(5), pp. 542–550, 2021, Apr. doi: 10.1038/s41592-021-01108-4.
