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Download the ChronosBH Data Sheet

ChronosBH is a time-domain fluorometer with picosecond resolution. Its optical design and automatic instrument control are state-of-the-art for time-resolved fluorometers.

ChronosBH is fully-automated through Vinci, a user-friendly, Windows-based software package.

Key features of ChronosBH include:

  • Flexible instrument configuration with a variety of light sources
  • A compact footprint and short optical path length for maximum sensitivity and efficient light coupling into the sample
  • Millisecond to picosecond lifetime measurement capabilities
  • Full automation of instrument components including: cuvette holder, polarizers, shutters, filterwheel, monochromators and stirrers
  • PC-controlled integration of temperature bath, titrator, stopped-flow apparatus and pressure pump
  • Upgradable to include steady-state measurements
  • T-format and parallel beam optical design for fast and precise polarization measurements

Key characteristics of data acquisition via Time-domain:

  • Is a more direct way of measuring lifetime
  • Requires no reference but measurement of an instrument response function (IRF)
  • Anisotropy decay measurements require two separate measurements at each plane of polarization
  • Has a low duty cycle, approximately only one photon per every 50 flashes is measured

Specifications for ChronosBH

Light Source Laser diodes (405, 436, 473, 635, 690, 780 and 830 nm), LEDs (280, 300, 335, 345, 460, 500, and 520 nm), and Ti:Sapphire, white and other pulsed lasers
Focusing & Collection Geometry Parallel beam design for precise polarization measurements
Polarizers UV grade Glan-Thompson with L/A=2.0
Detectors PMTs and MCPs
Detection Modes Fast analog and photon counting electronics
Wavelength Range 160 - 850 nm (MCP), 185 - 850 (PMT)
TCSPC Modules • Electrical Time Resolution down to 8 ps FWHM/5 ps rms
• Minimum Time Channel Width 820 fs
• Total useful count rate up to 4 MHz
• Measurement times down to 1 ms
Lifetime Measurements Range 10¹¹ sec to 10² sec
OS Requirements Windows XP operating system
Power Requirements Universal power input: 110-240 V, 50/60 Hz, 400 VAC
Dimensions 540 mm (L) x 425 mm (W) x 235 mm (H)
Weight 25 Kg

Schematic Diagram for ChronosBH

Measurement Examples from ChronosBH

Time-Domain Intensity Decay

Time-domain intensity decay of a paper sample acquired on ChronosBH using a 405-nm pulsed laser diode. The paper sample was placed in a front-surface accessory. The data is best fitted by a single exponential decay time of 0.94 ns (x2 = 1.25).

Time-Domain Anisotropy Decay

Time-domain anisotropy decays of Coumarin 6 in ethylene glycol acquired on ChronosBH using a 447-nm pulsed laser diode. The emission was collected through a KV 505 long high pass filter. The calculated value for θ = 2.6 ns with R0 = 0.38 and τ = 2.3 ns, T = 20-21°C.

Selected publications featuring ChronosBH

There are no publications currently available for ChronosBH. In the meantime, please consider reviewing the following Application Note:

Time-Domain Lifetime Measurements on ChronosBH
Yuhua Ni and Ewald Terpetschnig, ISS, Inc.

Accessories available for ChronosBH

The following accessories are available for ChronosBH. For more information please visit our Fluorescence Accessories page.

  • Laser Diodes &ammp; Light Emitting Diodes (Pulsed)
  • Fianum white light lasers
  • Lamp Mono-Assembly
  • Two-cuvette Sample Compartment
  • Three-cuvette Sample Compartment
  • Four-cuvette, Peltier-controlled, Sample Compartment
  • Dewar Flask
  • The HPCell™ High Pressure Cell System
  • Total Internal Reflection Fluorescence(TIRF) Flow Cell
  • Variable-angle, Front-Surface Sample Compartment
  • Vacuum Chamber
  • Microwell Plate Reader Accessory
  • UV Glan-Thompson Prism Polarizers
  • Titrators (1 and 2 syringe)
  • Stopped-Flow Devices
  • Fiber Optics
  • Computer-controlled Filter Wheels
  • Microchannel Plate Detector