ChronosBH is a time-domain fluorometer with picosecond resolution. Its optical design and automatic instrument control are state-of-the-art for time-resolved fluorometers.

ChronosBH is fully-automated through Vinci, a user-friendly, Windows-based software package.

Key features of ChronosBH include:

  • Flexible instrument configuration with a variety of light sources
  • A compact footprint and short optical path length for maximum sensitivity and efficient light coupling into the sample
  • Millisecond to picosecond lifetime measurement capabilities
  • Full automation of instrument components including: cuvette holder, polarizers, shutters, filterwheel, monochromators and stirrers
  • PC-controlled integration of temperature bath, titrator, stopped-flow apparatus and pressure pump
  • Upgradable to include steady-state measurements
  • T-format and parallel beam optical design for fast and precise polarization measurements

Specifications for ChronosBH

Light Source
  • Laser diodes (nm): 370, 405, 436, 473, 635, 690, 780, 830
  • LEDs (nm): 280, 300, 335, 345, 460, 500, 520
  • Pulsed Lasers: Supercontinuum, Ti:Sapphire, Pulsed Laser Diodes
Focusing & Collection Geometry Parallel beam design for precise polarization measurements
Polarizers UV grade Glan-Thompson with L/A=2.0
Detectors PMTs and MCPs
Detection Modes Fast analog and photon counting electronics
Wavelength Range 160 - 850 nm (MCP), 185 - 850 (PMT)
TCSPC Modules • Electrical Time Resolution down to 8 ps FWHM/5 ps rms
• Minimum Time Channel Width 820 fs
• Total useful count rate up to 4 MHz
• Measurement times down to 1 ms
Lifetime Measurements Range 10-11 sec to 10-2 sec
OS Requirements Windows 10
Power Requirements Universal power input: 110-240 V, 50/60 Hz, 400 VAC
Dimensions 540 mm (L) x 425 mm (W) x 235 mm (H)
Weight 25 kg

Schematic Diagram for ChronosBH

Measurement Examples from ChronosBH

Time-Domain Intensity Decay

Time-domain intensity decay of a paper sample acquired on ChronosBH using a 405-nm pulsed laser diode. The paper sample was placed in a front-surface accessory. The data is best fitted by a single exponential decay time of 0.94 ns (x2 = 1.25).

Time-Domain Anisotropy Decay

Time-domain anisotropy decays of Coumarin 6 in ethylene glycol acquired on ChronosBH using a 447-nm pulsed laser diode. The emission was collected through a KV 505 long high pass filter. The calculated value for θ = 2.6 ns with R0 = 0.38 and τ = 2.3 ns, T = 20-21°C.

Biochemistry & Molecular Biology (Membranes, Nucleic Acids, Proteins)

Hydroxymethylation of DNA Influences Nucleosomal Conformation and Stability in Vitro.
Mendonca, A., Chang, E.H., Liu, W., Yuan, C.
Biochim Biophys Acta., 2014, 1839(11), 1323-9.
Solution Scattering and FRET Studies on Nucleosomes Reveal DNA Unwrapping Effects of H3 and H4 Tail Removal.
Andresen, K., Jimenez-Useche, I., Howell, S.C., Yuan, C., Qiu, X.
PLoS One., 2013, 8(11), e78587.
DNA Methylation Regulated Nucleosome Dynamics.
Jimenez-Useche, I., Ke, J., Tian, Y., Shim, D., Howell, S.C., Qiu, X., Yuan, C.
Sci Rep., 2013, 3, 2121.
Clipping of Flexible Tails of Histones H3 and H4 Affects the Structure and Dynamics of the Nucleosome
Nurse, N.P., Jimenez-Useche, I., Smith, I.T., Yuan, C.
Biophys J., 2013, 104(5), 1081-8.
The Effect of DNA CpG Methylation on the Dynamic Conformation of a Nucleosome
Jimenez-Useche, I., Yuan, C.
Biophysical Journal, 2012, 103, 2502-2512.
Conformational Dynamics of Titin PEVK Explored with FRET Spectroscopy
Huber, T., Grama, L., Hetényi, C., Schay, G., Fülöp, L., Penke, B., Kellermayer, M.S.Z.
Biophysical Journal, 2012, 103(7), 1480-1489.


Lessons From Chlorophylls: Modifications of Porphyrinoids Towards Optimized Solar Energy Conversion.
Karcz, D., Boron, B., Matwijczuk, A., Furso, J., Staron, J., Ratuszna, A., Fiedor, L.
Molecules., 2014, 19(10), 15938-54.

Physical Chemistry

Photophysical Characterization of [Ir(ppy)2(dmb)][PF6] Towards Application in Light-Emitting Electrochemical Cells (LECs)
Zanoni, K.P., Sanematsu, M., Murakami Iha, N.Y.
Inorg Chem, 2014, 43, 162-164.
Solid State Molecular Device Based on a Rhenium(I) Polypyridyl Complex Immobilized on TiO2 Films
Patrocinio, A.O., Frin, K.P., Murakami Iha, N.Y.
Inorg. Chem., 2013, 52, 5889-5896.
A Pyrene Maleimide with a Flexible Linker for Sampling of Longer Inter-Thiol Distances by Excimer Formation
Niwayama, S., Kassar, A.S., Zhao, T., Sutton, R.B., Altenberg, G.A.
PLoS ONE, 2011, 6(10).


A Dual-Modality Optical Biopsy Approach for In Vivo Detection of Prostate Cancer in Rat Model
Sharma, V., Patel, N., Shen, J., Tang, L., Alexandrakis, G., Liu, H.
Journal of Innovative Optical Health Sciences, 2011, 4(3), 269-277.
Auto-fluorescence Lifetime and Light Reflectance Spectroscopy for Breast Cancer Diagnosis: Potential Tools for Intra-Operative Margin Detection
Sharma, V., Shivalingaiah, S., Peng., Y., Euhus, D., Gryczynski, Z., Liu, H.
Biomed Opt Express, 2012, 3(8), 1825-1840.

Accessories available for ChronosBH

The following accessories are available for ChronosBH. For more information please visit our Fluorescence Accessories page.

  • Laser Diodes &ammp; Light Emitting Diodes (Pulsed)
  • Fianum white light lasers
  • Lamp Mono-Assembly
  • Two-cuvette Sample Compartment
  • Three-cuvette Sample Compartment
  • Four-cuvette, Peltier-controlled, Sample Compartment
  • Dewar Flask
  • The HPCell™ High Pressure Cell System
  • Total Internal Reflection Fluorescence(TIRF) Flow Cell
  • Variable-angle, Front-Surface Sample Compartment
  • Vacuum Chamber
  • Microwell Plate Reader Accessory
  • UV Glan-Thompson Prism Polarizers
  • Titrators (1 and 2 syringe)
  • Stopped-Flow Devices
  • Fiber Optics
  • Computer-controlled Filter Wheels
  • Microchannel Plate Detector