Acquisition of an image from a zoomed area.

In this example, 1 nM Rhodamine110 solution was prepared in pH=8 buffer. The solution was placed in a well. After the buffer dried, the crystallized structure was measured with 60X magnification and a N.A. = 0.7 (air objective). The excitation wavelength was 488 nm with 1 W laser power; the emission was collected through a 50 μm pinhole.