Principles of Fluorescence Techniques

Every year ISS organizes a special workshop for students and researchers called the Principles of Fluorescence Techniques course which outlines the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies.

Topics addressed in this course include:
  •  Basic Definitions and Principles of Fluorescence
  •  Fluorescence Polarization
  •  Time-resolved Fluorescence
  •  Instrumentation
  •  Data Manipulation and Data Analysis
  •  Non-Linear Microscopy Including SHG
  •  GFP Fluorescence and Photoactivation
  •  Confocal and Multiphoton Fluorescence Microscopy
  •  FCS, Fluorescence Correlation Spectroscopy
  •  FLIM, Fluorescence Lifetime Imaging
  •  Single Molecule Imaging
  •  Image Processing and Deconvolution Approaches

To learn more about course locations and details, please visit the course website at