Achievements
Quality Policy
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Directions to ISS
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Gregorio Weber Award
Quality Policy
Career Opportunities
Contact Us
Directions to ISS
Comments & Suggestions
Gregorio Weber Award
06/06/2007: ISS Awarded NIH Grant
03/03/2007: ISS Announces 2007 Gregorio Weber Award Winner
02/18/2006: ISS Announces 2006 Gregorio Weber Award Winner
09/01/2005: ISS Achieves ISO 9001:2000 Certification
01/05/2005: ISS Announces a New Version of OxiplexTS™
12/23/2004: ISS Introduces Light Emitting Diodes (LEDs) for the Study of Proteins
News Archive
03/03/2007: ISS Announces 2007 Gregorio Weber Award Winner
02/18/2006: ISS Announces 2006 Gregorio Weber Award Winner
09/01/2005: ISS Achieves ISO 9001:2000 Certification
01/05/2005: ISS Announces a New Version of OxiplexTS™
12/23/2004: ISS Introduces Light Emitting Diodes (LEDs) for the Study of Proteins
News Archive
Fluorescence Products
Alba FCS: Fluorescence Correlation SpectrometerAlba FLIM: Fluorescence Lifetime Imaging
PC1: Photon Counting Spectrofluorimeter
K2: Multifrequency Cross-Correlation Phase and Modulation Fluorometer
Chronos: Frequency-Domain Lifetime Spectrometer
ChronosBH: Time-Domain Lifetime Spectrometer
Modular Components for Building Spectrofluorometers
Phoenix: SLM Upgrade Packages
Software
Request Literature for Fluorescence Products
Biomedical Products
OxiplexTSImagent
Request Literature for Biomedical Products
Fluorescence Quantum Yield Standards
Fluorescence Lifetime Standards
Lifetime Data of Selected Fluorophores
Excitation and Emission Wavelengths of Fluorophores
Fluorescent Probes Data Table
Fluorescence Standards for LEDs and Laser Diodes
Application & Technical Notes
Measurement Examples
Selected Publications
Laboratories & Societies
Fluorescence Lifetime Standards
Lifetime Data of Selected Fluorophores
Excitation and Emission Wavelengths of Fluorophores
Fluorescent Probes Data Table
Fluorescence Standards for LEDs and Laser Diodes
Application & Technical Notes
Measurement Examples
Selected Publications
Laboratories & Societies
Excitation and Emission Wavelengths of Fluorophores
The following is a list of excitation and emission wavelengths of probes utilized in fluorescence research and applications. The positions of the maxima depend upon the solvent utilized in the measurements; the table is only intended to provide an indication of the wavelength range covered by each fluorophore.
| Fluorophore | Excitation [nm] | Emission [nm] |
| 5-Hydroxytryptamine (HAT) | 370-415 | 520-540 |
| Acridine yellow | 470 | 550 |
| Acridine orange | 500 | 530 |
| Alexa Fluor 488 | 494 | 519 |
| Alexa Fluor 532 | 530 | 555 |
| Alexa Fluor 546 | 554 | 570 |
| BODIPY 500/510 | 508 | 515 |
| BODIPY 530/550 | 534 | 554 |
| Cascade Blue | 375 | 410 |
| Coumarin | 384 | 470 |
| CY2 | 489 | 506 |
| CY3 | 548 | 562 |
| CY5 | 650 | 670-700 |
| Dansyl | 340 | 520 |
| DAPI | 345 | 458 |
| DPH | 354 | 430 |
| Erythrosin | 529 | 554 |
| Ethidium Bromide | 510 | 595 |
| FITC | 494 | 518 |
| Fluorescein | 495 | 517 |
| FURA-2 | 340/380 | 500/530 |
| GFP | 395/489 | 509 |
| Hoechst 33258 | 365 | 480 |
| Hoechst 33342 | 355 | 465 |
| Laurdan | 364 | 497 |
| Lucifer yellow CH | 428 | 535 |
| Nile Red | 485 | 525 |
| Oregon Green 488 | 493 | 520 |
| Oregon Green 500 | 503 | 522 |
| Oregon Green 514 | 511 | 530 |
| Prodan | 361 | 498 |
| Pyrene | 341 | 376 |
| Rhodamine 110 | 496 | 520 |
| Rhodamine 123 | 505 | 534 |
| Rhodamine 6G | 525 | 555 |
| Rhodamine B | 540 | 625 |
| SITS | 336 | 438 |
| SNARF | 480 | 600/650 |
| Stilbene SITS, SITA | 365 | 460 |
| Texas Red | 589 | 615 |
| TOTO-1 | 514 | 533 |
| YOYO-1 | 491 | 509 |
| YOYO-3 | 612 | 631 |