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Data Tables

Fluorescence Standards for LEDs and Laser Diodes

The following data tables provide the names and lifetimes of lifetime standards that are recommended for use with LEDs and laser diodes listed below.

LEDs

Center Wavelength [nm] Standard Solvent Lifetime [ns]
280 P-TerphenylEthanol1.05
PPOEthanol1.46
295 P-TerphenylEthanol1.05
PPOEthanol1.46
300 P-TerphenylEthanol1.05
PPOEthanol1.46
330 Dimethyl-POPOPEthanol1.45
BBOEthanol1.24
370 Dimethyl-POPOPEthanol1.45
BBOEthanol1.24
460 FluoresceinPBS4
BodipyFLWater5.8
480 FluoresceinPBS4
BodipyFLWater5.8
520 Rhodamine BWater1.7
Rhodamine 590Water4.1

PBS = phosphate-buffered saline
POPOP = 1,4-bis(5-phenyloxazole-2-yl)-benzene
PPO = 2,5-diphenyl-oxazole
BBO = 2,5-bis([1,1'-biphenyl]-4-yl)-oxazole

Laser Diodes

Center Wavelength [nm] Standard Solvent Lifetime [ns]
370 Dimethyl-POPOPEthanol1.45
BBOEthanol1.24
405 Coumarin 6Ethanol2.5
Dimethyl-POPOPEthanol1.45
435 Coumarin 6Ethanol2.5
FluoresceinPBS4
470 FluoresceinPBS4
BodipyFLWater5.8
635 Cy5Water1
Alexa Fluor 647Water1
680 Alexa Fluor 700Water1
Alexa Fluor 750Water0.66
780 Indocyanine GreenWater0.52