Data Tables
Fluorescent Probes
The following data table contains the mean lifetimes, absorption and emission maxima of the free and bound forms of important fluorescent probes for ion recognition.
| Fluorescent Probes | Mean Lifetime [ns] | Absorption Max [nm] | Emission Max [nm] | |||
|---|---|---|---|---|---|---|
| free | bound | free | bound | free | bound | |
| a) Calcium Probes | ||||||
| Fura-2 | 1.09 | 1.68 | 362 | 335 | 500 | 503 |
| Indo-1 | 1.4 | 1.66 | 349 | 331 | 482 | 398 |
| Ca-Green | 0.92 | 3.66 | 506 | 506 | 534 | 534 |
| Ca-Orange | 1.20 | 2.31 | 555 | 555 | 576 | 576 |
| Ca-Crimson | 2.55 | 4.11 | 588 | 588 | 610 | 612 |
| Quin-2 | 1.35 | 11.6 | 356 | 336 | 500 | 503 |
| b) Magnesium Probes | ||||||
| Mg-Quin-2 | 0.84 | 8.16 | 353 | 337 | 487 | 493 |
| Mg-Green | 1.21 | 3.63 | 506 | 506 | 532 | 532 |
| c) Potassium Probe | ||||||
| PBFI | 0.52 | 0.59 | 350 | 344 | 546 | 504 |
| d) Sodium Probe | ||||||
| Sodium Green | 1.13 | 2.39 | 507 | 507 | 532 | 532 |
| e) pH Probes | ||||||
| SNAFL-1 | 1.19 | 3.74 | 539 | 510 | 616 | 542 |
| Carboxy-SNAFL-1 | 1.11 | 3.67 | 540 | 508 | 623 | 543 |
| Carboxy-SNAFL-2 | 0.94 | 4.60 | 547 | 514 | 623 | 545 |
| Carboxy-SNARF-1 | 1.51 | 0.52 | 576 | 549 | 638 | 585 |
| Carboxy-SNARF-2 | 1.55 | 0.33 | 579 | 552 | 633 | 583 |
| Carboxy-SNARF-6 | 1.03 | 4.51 | 557 | 524 | 635 | 559 |
| Carboxy-SNARF-X | 2.59 | 1.79 | 575 | 570 | 630 | 600 |
| Resorufin | 2.92 | 0.45 | 571 | 484 | 587 | 578 |
| BCECF | 4.49 | 3.17 | 503 | 484 | 528 | 514 |
References:
- Topics in Fluorescence Spectroscopy (Vol. 4): Probe Design and Chemical Sensing, J.R. Lakowicz (Editor), Plenum Press, New York and London, 1994.
