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Data Tables

Fluorescent Probes

The following data table contains the mean lifetimes, absorption and emission maxima of the free and bound forms of important fluorescent probes for ion recognition.

Fluorescent Probes Mean Lifetime [ns] Absorption Max [nm] Emission Max [nm]
  free bound free bound free bound
a) Calcium Probes  
Fura-2 1.09 1.68 362 335 500 503
Indo-1 1.4 1.66 349 331 482 398
Ca-Green 0.92 3.66 506 506 534 534
Ca-Orange 1.20 2.31 555 555 576 576
Ca-Crimson 2.55 4.11 588 588 610 612
Quin-2 1.35 11.6 356 336 500 503
 
b) Magnesium Probes  
Mg-Quin-2 0.84 8.16 353 337 487 493
Mg-Green 1.21 3.63 506 506 532 532
 
c) Potassium Probe  
PBFI 0.52 0.59 350 344 546 504
 
d) Sodium Probe  
Sodium Green 1.13 2.39 507 507 532 532
 
e) pH Probes  
SNAFL-1 1.19 3.74 539 510 616 542
Carboxy-SNAFL-1 1.11 3.67 540 508 623 543
Carboxy-SNAFL-2 0.94 4.60 547 514 623 545
Carboxy-SNARF-1 1.51 0.52 576 549 638 585
Carboxy-SNARF-2 1.55 0.33 579 552 633 583
Carboxy-SNARF-6 1.03 4.51 557 524 635 559
Carboxy-SNARF-X 2.59 1.79 575 570 630 600
Resorufin 2.92 0.45 571 484 587 578
BCECF 4.49 3.17 503 484 528 514

References:

  1. Topics in Fluorescence Spectroscopy (Vol. 4): Probe Design and Chemical Sensing, J.R. Lakowicz (Editor), Plenum Press, New York and London, 1994.