PC1™ Specifications

Measurements (Steady-State Fluorescence)

• Corrected excitation and emission spectra
• Excitation-emission matrices
• Polarization (anisotropy) measurements
• Millisecond kinetics in photon counting mode
• Dual-wavelength-ratiometric excitation or emission measurements

Light Sources

• 300 W high-pressure xenon arc lamp, 45 mW/nm brightness at 275 nm
• Lamp power supply: controllable in current, with time meter

Optional Sources

• Laser diodes
• Light emitting diodes (LEDs)
• Continuous wave lasers (argon-ion, krypton-ion, helium-cadmium, etc.)

Monochromators

• Single concave holographic grating

  Wavelength range: from 200 nm to 1200 nm (dependent on selected grating)
  Wavelength accuracy: ±0.2 nm
  Wavelength reproducibility: ±0.25 nm
  Slew rate: 160 nm/s

• Optional: double grating

Lenses

• UV-grade fused silica lenses

Polarizers

• UV-grade Glan-Thompson, 10x10 mm, L/A=2.0
• UV-grade Glan-Thompson, 14x14 mm, L/A=2.0
• UV-grade Glan-Taylor, 10x10 mm, L/A=2.0 (for high power lasers)

Optical Design and Collection Geometry

• Parallel beam design for precise polarization measurements
• T-format for simultaneous acquisition on 2 emission channels

Detectors

• Selected side-on photomultiplier tubes in room-temperature or cooled housing
• Optional: CCD camera
• Emission channels PMTs: Model R928P by Hamamatsu
• Reference detector PMT: Model R928 by Hamamatsu
• PMT wavelength range: 240-900 nm

Detection Modes

• Photon counting electronics, 10 KHz, on 3 independent channels
• Optional: Analog Output

Pre-Amplifier Discriminators

• 80 MHz bandwidth, TTL output

Dynamic Range

• Linear up to 4 million counts/second

Sensitivity

• 800 fM of fluorescein (with cooled PMT housing)

Signal-to-Noise Ratio

• 2000:1 (room temperature PMT housing)
• 6000:1 (cooled PMT housing)

Automation

• Control of 4 shutters
• Up to 3 monochromators
• 3 polarizers
• Sample holder rotation
• Stirrers
• Filterwheel

Instrument Interface to RS232 Devices

• Titrators
• Stopped-flow apparatus
• Peltier sample compartment
• Flow-through temperature bath

Computer and Operating System

• Intel-type CPU, Windows XP operating system

Power Requirements

• Universal power input: 110-240 V, 50/60 Hz, 400 VAC

Dimensions

• 885 mm (L) x 600 mm (W) x 330 mm (H)
• With lamp: 885 mm (L) x 835 mm (W) x 330 mm (H)

Weight

• 40 kg

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