Vinci Specifications

Instrument Control

• Automation of ISS instrument components
• Control of external devices
  • Temperature bath (Neslab, Fisher)
  • Titrator (ISS, Hamilton)
  • Stopped-flow apparatus (Molecular Kinetics, OLIS, Hi-Tech, Applied Photophysics, Kintek)
  • Pressure pump
• Recording from temperature/pressure sensors
• Storage of all experimental parameters in the experiment data file

Data Acquisition

Vinci supports one-dimensional, multi-dimensional and user-defined protocols

• One-dimensional measurements
  • Corrected Excitations and emission spectra
  • Excitation-Emission matrices
  • Polarization/Anisotropy
  • Kinetics (slow, fast)
  • Titrations
  • Fluorescence decay times (exponential and non-exponential)
  • Rotational correlation times (isotropic, anisotropic, hindered)
  • Time-resolved spectra
  • Phase- and modulation-resolved spectra
• Multi-dimensional measurements

  Vinci supports multi-dimensional data acquisition; the user can select from any of the following experimental combinations:

  Fluorescence Intensity Polarization/Anisotropy Ratiometric Measurements	vs.	Excitation (Emission) Wavelength and/or any of the following: Emission (Excitation) Wavelength Sample Concentration Sample Temperature Sample Pressure
  Fluorescence Intensity Polarization/Anisotropy Ratiometric Measurements	vs.	Time and/or any of the following: Excitation Wavelength Emission Wavelength Sample Concentration Sample Temperature Sample Pressure
  Phase and Modulation Differential Polarization	vs.	Modulation Frequency and/or any of the following: Excitation Wavelength Emission Wavelength Sample Concentration Sample Temperature Sample Pressure

• User defined protocols

The user can define protocols for both one-dimensional and multi-dimensional measurements. Protocols can be stored and rerun.

Data Analysis

Spectral Analysis

• Normalization
• Smoothing (average, median, Savitzky-Golay)
• Derivative (nth-order)
• Integration (area, average and standard deviation)
• Spectral correction
• Spectral moments
• Conversion to wave numbers
• Data Interpolation
• User-defined filtering
• Fitting to user-defined model

Spectral Operations

• Addition, subtraction, multiplication, division
• Scaling factor

Time-Resolved Analysis

• Multiple decays for up to 4 components for exponential, non exponential and lifetime distributions
• Rotational correlation times for isotropic, anisotropic and hindered rotators
• User-defined models
• Phase- and modulation-resolved spectra
• Time-resolved spectra

Simulation

• Phase and demodulation versus modulation frequency for up to 4 components for exponential, non exponential and lifetime distributions
• Differential phase and modulation ration versus modulation frequency for isotropic, anisotropic and hindered rotators

Display

• 2D and 3D display with user-defined colors and fonts
• 3D surface rotation and in/out zooming
• Cursor identification of XY spectra coordinates

Data Format

• The data files are stored in an accessible text (ASCII) format.
• The experiment data file, or raw data file, includes the information about the experiment protocol and, as such, it can be re-loaded to rerun the original experiment.
• Experiment data files cannot be overwritten; once an experiment data file is modified, the new file is stored as analysis data file. Experiment files and Analysis files are identifiable through their different extensions and icons.

Data and Plots Export

• Plots export to gif, tiff, jpeg, png, bitmaps and metafiles
• Compatible with LFD and CFS formats

Links

• To Global Analysis by Globals Unlimited
• To DATAN by MultiD Analysis AB

Import

• ISS (cat, lif, pol), LFD, SLM

Operating System Supported

• Windows 2000, Windows XP

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