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Alba FLIM™

Confocal Lifetime Imaging

ISS Alba FLIM™


Alba FLIM™ is a computer-controlled instrument specifically designed for confocal imaging applications using either single- or multi-photon excitation. The modular design of the instrument allows it to be easily upgraded to acquire Fluorescence Correlation Spectroscopy (FCS) data. Besides its most attractive features such as high sensitivity and fast acquisition speed, Alba FLIM™ comes with Vista, a powerful and user-friendly data acquisition and analysis software package.

Alba FLIM™:

Instrument Features
  • Dual channel acquisition
  • Separate pinholes for each channel for higher resolution
  • Computer-controlled alignment of the confocal pinhole and optics
  • Choice between scanning mirrors or piezo-controlled XYZ stage
  • Single- and multi-photon excitation
  • Powered by Vista, a user-friendly software package for the acquisition of FLIM, FRET, RICS, and FCS
  • Alba FLIM™ can be combined with Alba FCS™ to a powerful, fully-equipped Alba - Confocal Spectroscopy and Imaging Workstation
Measurements
  • Fluorescence Lifetime Images (FLIM)
  • Fluorescence Resonance Energy Transfer (FRET) efficiency images
  • Fluorescence images
  • Raster imaging correlation spectroscopy (RICS)
  • Steady-state polarization and ratio imaging
Excitation Modality

One-photon (using an argon ion, krypton ion, or dye-laser) or multi-photon measurements (using a Ti:Sapphire laser) are possible with Alba FLIM™.

Microscopes

Alba FLIM™ can be interfaced with either a Nikon or an Olympus epifluorescence microscope.

Detectors

Standard light detectors are cooled photomultiplier tubes.

Upgradeable to Alba FCS™

Alba FLIM™ can be upgraded to acquire FCS data. All in a single instrument!