Alba FCS™ Specifications

Autocorrelation and Cross-Correlation Functions:
Vista resolves 1 or 2 species using:
- Diffusion coefficient
- Diffusion time
- Sample concentration
- Triplet state decay time constant
- Triplet function
- Flow rate
- Size of excitation volume
- Number of molecules
Photon Counting Histogram (PCH):
Vista resolves 1 or 2 species using:
- Number of molecules
- Molecular brightness

Light Sources:
- Single photon lasers housed in a laser launcher with computer-control of beam expander, laser intensity and shutter; or,
- Multi-photon excitation with computer-control of beam expander, laser intensity and shutter
Optics:
- An adapted Nikon microscope with lens revolver for objectives, bottom and side ports and computer-controlled XY-stage for sample slides and for 384-well plates.
- Available for Leica, Olympus and Zeiss inverted and upright microscopes
- Objectives:
  - Air objectives with 20X, 40X, 60X magnification and 1.5-8.1 working distances
  - Oil immersion objectives, 1.4 NA and 60X (standard); other aperture available
  - Water immersion objectives,1.2 NA 60X (standard), with coverslip correction (for 0.15-0.18 coverslip); other apertures available
- Dichroic Filters:
  - For single-photon excitation: 1-, 2-, 3-band filters
  - For multi-photon excitation
- Polarizer:
  - Cube beam splitter, wavelength range: 450-1100 nm; extinction ratio: 10,000:1 at +/- 3degrees
Stage:
- Large distance movement (100x100x10 mm)
  - Stepper motor-controlled XYZ stage
- Micro-distance movements
  - XYZ piezo-controlled stage, 100x100x50 µm with 5 nm step resolution.
Sample Holders
- Microwell plates
- Petri dishes
- Coverslip
Light Detectors:
- Both detectors feature pre-amplifier discriminators with TTL output avalanche photodiodes (APDs)
- Cooled photomultiplier tubes

Operating System
- Windows ME/XP
Data Acquisition Modes
- Time mode
- Photon mode
Statistical Functions Utilized for Data Analysis
- Autocorrelation function
- Cross-correlation function
- Photon Counting Histogram (PCH)
Single Set and Global Fitting Models for Fitting
- Autocorrelation and cross-correlation functions
  Vista resolves 1 or 2 species using:
  - 2D- or 3D-Gaussian PSF
  - 3D-Gaussian-Lorentzian PSF
  - One-photon excitation
  - Two-photon excitation
  - Presence of flow
  - Input of user-defined equation
- Photon Counting Histogram (PCH)
  Vista resolves 1 or 2 species using:
  - 2D- or 3D uniform
  - 2D- or 3D-Gaussian PSF
  - 3D-Gaussian-Lorentzian PSF
  - One-photon excitation
  - Two-photon excitation