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06/06/2007: ISS Awarded NIH Grant
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02/18/2006: ISS Announces 2006 Gregorio Weber Award Winner
09/01/2005: ISS Achieves ISO 9001:2000 Certification
01/05/2005: ISS Announces a New Version of OxiplexTS™
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Fluorescence Products
Alba FCS: Fluorescence Correlation SpectrometerAlba FLIM: Fluorescence Lifetime Imaging
PC1: Photon Counting Spectrofluorimeter
K2: Multifrequency Cross-Correlation Phase and Modulation Fluorometer
Chronos: Frequency-Domain Lifetime Spectrometer
ChronosBH: Time-Domain Lifetime Spectrometer
Modular Components for Building Spectrofluorometers
Phoenix: SLM Upgrade Packages
Software
Request Literature for Fluorescence Products
Biomedical Products
OxiplexTSImagent
Request Literature for Biomedical Products
Fluorescence Lifetime Standards
Lifetime Data of Selected Fluorophores
Excitation and Emission Wavelengths of Fluorophores
Fluorescent Probes Data Table
Fluorescence Standards for LEDs and Laser Diodes
Application & Technical Notes
Measurement Examples
Selected Publications
Laboratories & Societies
Selected References for the Use of Alba™ in Various Research Areas
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Basics
Time-Integrated Fluorescence Cumulant Analysis in Fluorescence Fluctuation Spectroscopy
Wu, B., Müller, J.D.
Biophys. J., 2005, 89, 2721–2735.3-D Particle Tracking in a Two-Photon Microscope: Application to the Study of Molecular Dynamics in Cells
Levi, V., Ruan, Q., Gratton, E.
Biophys. J., 2005, 88, 2919–2928.Position-Sensitive Scanning Fluorescence Correlation Spectroscopy
Skinner, J.P., Chen, Y., Müller, J.D.
Biophys. J., 2005, 89, 1288–1301.Chromatin Dynamics in Interphase Cells Revealed by Tracking in a Two-Photon Excitation Microscope
Levi, V., Ruan, Q., Plutz, M., Belmont, A.S., Gratton, E.
Biophys. J., 2005, 89, 4275–4285.Molecular Brightness Determined from a Generalized Form of Mandel’s Q-Parameter
Sanchez-Andres, A., Chen, Y., Müller, J.D.
Biophys. J., 2005, 89, 3531–3547.High-Pressure Cell for Fluorescence Fluctuation Spectroscopy
Tekmen, M., Müller, J.D.
Rev. Sci. Instrum., 2004, 75(12), 5143-5148.Cumulant Analysis in Fluorescence Fluctuation Spectroscopy
Müller, J.D.
Biophys. J., 2004, 86, 3981–3992.The Photon Counting Histogram in Fluorescence Fluctuation Spectroscopy with Non-Ideal Photodetectors
Hillesheim, L.N., Müller, J.D.
Biophys. J., 2003, 85, 1948–1958.An Integrated Platform for Surface Forces Measurements and Fluorescence Correlation Spectroscopy
Mukhopadhyay, A., Zhao, J., Bae, S.C, Granicka, S.
Rev. Sci. Instrum., 2003, 74(6), 3067-3071.Near Infrared Microspectroscopy, Fluorescence Microspectroscopy, Infrared Chemical Imaging and High-Resolution Nuclear Magnetic Resonance Analysis of Soybean Seeds, Somatic Embryos and Single Cells
Baianu, I.C., Costescu, D., You, T., Lozano, P.R., Hofmann, N.E., Korban, S.S.
AOCS Symp. Proceed., 2003, 94(12).Trapped Brownian Motion in Single- and Two-Photon Excitation Fluorescence Correlation Experiments
Chirico, G., Fumagalli, C., Baldini, G.
J. Phys. Chem., 2002, 106, 2508-2519.Two-Photon Microscopy and Spectroscopy Based on a Compact Confocal Scanning Head
Diaspro, A., Chirico, G., Federici, F., Cannone, C., Beretta, S., Robello, M.
J. of Biomed. Opt., 2001, 6(3), 300–310. -
Biochemistry
Applications of Dual-Color Fluorescence Cross-Correlation Spectroscopy in Antibody Binding Studies
Ruan, Q., Tetin, S.Y.
Anal. Biochem., 2008.Fructose-1,6-bisphosphate Acts Both as an Inducer and as a Structural Cofactor of the Central Glycolytic Genes Repressor (CggR)
Zorrilla, S., Chaix, D., Ortega, A., Alfonso, C., Doan, T., Margeat, E., Rivas, G., Aymerich, S., Declerck, N., Royer, C.A.
Biochemistry, 2007, 46(51), 14996-15008.Monomer Dimer Dynamics and Distribution of GPI-Anchored uPAR are Determined by Cell Surface Protein Assemblies
Caiolfa, V.R., Zamai1, M., Malengo, G., Andolfo, A., Madsen, C.D., Sutin, J., Digman, M.A., Gratton, E., Blasi1, F., Sidenius, N.
J. Cell Biol., 2007, 179, 1067-1082.Escherichia coli Ribosomal Protein L20 Binds as a Single Monomer to its Own mRNA Bearing Two Potential Binding Sites
Allemand, F., Haentjens, J., Chiaruttini, C., Royer, C., Springer, M.
Nucleic Acids Research, 2007, 35(9), 3016-3031.Mobility and Distribution of Replication Protein A in Living Cells at Single Molecule Level
Braet, C., Stephan, H., Dobbie, I., Togashi, D., Ryder, A.G., Földes-Papp, Z., Lowndes, N., Nasheuer., H.P.
Experimental & Molecular Pathology, 2007, 82(2), 156-62.Interactions of Two Monoclonal Antibodies with BNP: High Resolution Epitope Mapping Using Fluorescence Correlation Spectroscopy
Tetin, S.Y., Ruan, Q., Saldana, S.C., Pope, M.R., Chen, Y., Wu, H., Pinkus, M.S., Jiang, J., Richardson, P.L.
Biochemistry, 2006, 45(47), 14155-65.Quantitative Detection of the Ligand-dependent Interaction Between the Androgen Receptor and the Co-activator, Tif2, in Live Cells Using Two Color, Two Photon Fluorescence Cross-correlation Spectroscopy
Rosales, T., Georget, V., Malide, D., Smirnov, A., Xu, J., Combs C., Knutson, J.R., Nicolas, J.-C., Royer, C.A.
Eur. Biophys. J., 2006.The Nuclear Receptor Coactivator PGC-1α Exhibits Modes of Interaction with the Estrogen Receptor Distinct From those of SRC-1
Bourdoncle, A., Labesse, G., Margueron, R., Castet, A., Cavaillès, V., Royer, C.A.
J. Mol. Biol., 2005, 347, 921-934.Phosphoinositide Specificity of and Mechanism of Lipid Domain Formation by Annexin A2-p11 Heterotetramer
Gokhale, N.A., Abraham, A., Digman, M.A., Gratton, E., Cho, W.
J. Biol. Chem., 2005, 280(52), 42831-42840.The Nuclear Receptor Coactivator PGC-1a Exhibits Modes of Interaction with the Estrogen Receptor Distinct from Those of SRC-1
Bourdoncle, A., Labesse, G., Margueron, R., Castet, A., Cavaillès, V., Royer, C.A.
J. Mol. Biol., 2005, 347(5), 921-934.Vesicle Encapsulation Studies Reveal that Single Molecule Ribozyme Heterogeneities Are Intrinsic
Okumus, B., Wilson, T.J., Lilley, D.M.J., Ha, T.
Biophys. J., 2004, 87, 2798–2806.Direct Gene Expression Analysis
Winter, H., Korn, K., Rigler, R.
Current Pharmaceutical Biotechnology, 2004, 5, 191-197.Pleckstrin Homology Domain Diffusion in Dictyostelium Cytoplasm Studied Using Fluorescence Correlation Spectroscopy
Ruchira, Hink, M.A., Bosgraaf, L., Van Haastert, P.J.M., Visser, A.J.W.G.
The Journal of Biological Chemistry, 2004, 279(11), 10013–10019.Polarized Fluorescence Correlation Spectroscopy of DNA-DAPI Complexes
Barcellona, M.L., Gammon, S., Hazlett, T., Digman, M.A., Gratton, E.
Microscopy Research and Techniques, 2004, 65, 205-217.Segregation of Saturated Chain Lipids in Pulmonary Surfactant Films and Bilayers
Nag, K., Pao, J.-S., Harbottle, R.R., Possmayer, F., Petersen, N.O., Bagatolli, L.A.
Biophys. J., 2002, 82, 2041–2051.Molecular Heterogeneity of O-Acetylserine Sulfhydrylase by Two-Photon Excited Fluorescence Fluctuation Spectroscopy
Chirico, G., Bettati, S., Mozzarelli, A., Chen, Y., Müller, J.D., Gratton, E.
Biophys. J., 2001, 80, 1973–1985. -
Sensing
Fluorescence Correlation Spectroscopy Assay for Gliadin in Food
Varriale, A., Rossi, M., Staiano, M., Terpetschnig, E., Barbieri, B., Rossi, M., D'Auria, S.
Anal Chem. 2007, 79(12), 4687-4689. -
Diagnostics
Application of Fluorescence Correlation Spectroscopy to Hapten-Antibody Binding
Hazlett, T.L., Ruan, Q., Tetin, S.Y.
Methods in Molecular Biology, 2005, 305, 415-438.Antibodies in Diagnostic Applications
Tetin, S.Y., Stroupe, S.D.
Current Pharmaceutical Biotechnology, 2004, 5, 9-16. -
Surface Physics
How Confined Lubricants Diffuse During Shear
Mukhopadyay, A., Bae, S.C., Zhao, J., Granick, S.
Physical Review Letters, 2004, 93, 236105.Polymer Lateral Diffusion at the Solid-Liquid Interface
Zhao, J., Granick, S.
J. Am. Chem. Soc., 2004, 126, 6242-6243.Slaved Diffusion in Phospholipid Bilayers
Zhang, L., Granick, S.
PNAS, 2005(102), 26, 9118-9121.Integrated Platform for Surface Forces Measurements and Fluorescence Correlation Spectroscopy
Mukhopadyay, A., Zhao, J., Bae, S.C., Granick, S.
Rev. Sci. Inst., 2003, 74, 3067. -
Nanomaterials
How to Stabilize Phospholipid Liposomes (Using Nanoparticles)
Zhang, L., Granick, S.
Nano Lett., 2006(6), 4, 694-698. -
Photon Counting Histogram
Unraveling Protein-Protein Interactions in Living Cells with Fluorescence Fluctuation Brightness Analysis
Chen, Y., Wei, L.-N., Müller, J.D.
Biophys. J., 2005, 88, 4366–4377.Dual-Color Photon-Counting Histogram
Chen, Y., Tekmen, M., Hillesheim, L., Skinner, J., Wu, B., Müller, J.D.
Biophys. J., 2005, 88, 2177–2192.Fluorescence Excitation Volume in Two-Photon Microscopy by Autocorrelation Spectroscopy and Photon Counting Histogram
Chirico, G., Olivini, F., Beretta, S.
Applied Spectroscopy, 2000, 54, 1084-1090. -
Data Acquisition System and Software
Data Acquisition Card for Fluctuation Correlation Spectroscopy Allowing Full Access to the Detected Photon Sequence
Eid, J.S., Müller, J.D., Gratton, E.
Rev. Sci. Instr., 2000, 71, 361-368. -
FLIM, Fluorescence Lifetime Imaging
Fluorescence Lifetime Imaging Study of a Thin Protein Layer on Solid Surfaces
Togashi, D.M., Ryder, A.G.
Experimental & Molecular Pathology, 2007, 82(2), 135-41.

