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Fluorescence Products
Alba FCS: Fluorescence Correlation SpectrometerAlba FLIM: Fluorescence Lifetime Imaging
PC1: Photon Counting Spectrofluorimeter
K2: Multifrequency Cross-Correlation Phase and Modulation Fluorometer
Chronos: Frequency-Domain Lifetime Spectrometer
ChronosBH: Time-Domain Lifetime Spectrometer
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Phoenix: SLM Upgrade Packages
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OxiplexTSImagent
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Fluorescence Lifetime Standards
Lifetime Data of Selected Fluorophores
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Alba FCS™
Fluorescence Correlation Spectrometer
Alba FCS™ is a dual channel spectrometer that combines a confocal scanning microscope with
fluorescence correlation spectroscopy (FCS) and is optimized for both single-photon
and multi-photon excitation.
Alba FCS™, Spectroscopy on Single Molecules
Applications
- Measurement of molecular dynamics
- Measurement of kinetic rate constants
- Antibody-antigen interactions
- Ligand-receptor interactions
- Nucleic acid hybridization
- Molecular aggregation and polydispersity
- Chemical reactions
Measurement Parameters
a) When using autocorrelation and cross-correlation functions:
- Diffusion coefficient
- Diffusion time
- Concentration
- Triplet state decay time constant
- Triplet function
- Flow rate
- Size of excitation volume
- Number of molecules
b) When using Photon Counting Histogram (PCH):
- Number of molecules
- Molecular brightness
Acquisition Modes
Alba acquires either in time-mode (the classical way: photons acquired in time intervals are counted) or in photon mode (time delay between one photon and the next one is measured and an histogram is built). A dual-channel data acquisition card receives the data stream from the detectors and passes them on to the computer.
Raw Data Stream
Raw data are stored in a data file for further processing. No need anymore to reject an autocorrelation function because of large particle interference. Raw data can be purged from large particles presence and the autocorrelation function can be recalculated.
Excitation Modality
Single photon excitation (using an argon ion, krypton ion, or dye-laser); or multi-photon excitation (using a Ti:Sapphire laser).
Microscopes
Alba can be interfaced to either an Olympus or a Nikon epifluorescence microscope.
Detectors
Light detectors are cooled photomultiplier tubes or avalanche photodiodes. The system is provided for single channel or dual channel data acquisition for cross-correlation measurements.
Alba FCS™ can be combined with Alba FLIM™ to a powerful, fully-equipped Alba - Confocal Spectroscopy and Imaging Workstation.