Imaging can be achieved by any of two ways:
- Using the galvo-controlled mirrors: the laser beam is scanned over the sample following a predetermined pattern (laser scanning microscope). This is achieved by using galvo-controlled mirrors that scan the beam on the XY-plane. The z-axis change is achieved by mounting the objective on a piezo-stage, or using a stage with piezo z-axis control
- Using a XYZ piezo-controlled stage; the beam is set at a position while the sample is moved over the beam (stage moving microscope).
Imaging Options: Which one should be used?
The user can select one of the two options, or they can be implemented at the same time on the instrument.
The galvo-controlled mirrors cover a surface area of about 200 Ám in diameter with no optical distortions in the image. Image acquisition is fast (up to 4 Ás dwell time per pixel). This option is utilized for fast imaging acquisition, fast scanning FCS, particle tracking and RICS acquisition.
The XYZ piezo-controlled stage can be utilized on Alba. When using this option, the laser beam stands still while the sample is moved. The piezo can scan the sample over an area of 300 x 300 Ám2 or higher with no distortions in the imaging capabilities.